Oral Presentations

Ryan J. Rafferty and Richard M. Hyslop, “Investigation into the toxicity of 6-thiopurine: Inhibition of 6TP and metabolites of UDPGDH and development of methodologies for the assessment of toxicity” HPLC Symposium 2005,  Stockholm, Sweeden, June 2005  (Invited Speaker, unable to attend).

Ryan J. Rafferty, “Toxicology of 6-Thiopurine.”  University of Northern Colorado, Greeley, CO, April 2004  (Invited Speaker to Toxicology Course).

Ryan J. Rafferty, “Operational Laboratory Safety.”  University of Northern Colorado, Greeley, CO, August 2003.

Ryan J. Rafferty and Richard M. Hyslop, “Analytical Methodology for the Analysis of UDP-Glucose Dehydrogenase Activity by High Performance Liquid Chromatography-Mass Spectrometry.”  Colorado-Wyoming Academy of Sciences, University of Northern Colorado, April 2003.

Ryan J. Rafferty and Richard M. Hyslop, “Analysis of Purine Analog Inhibitory Activity Towards UDP-Glucose Dehydrogenase.”  Colorado-Wyoming Academy of Sciences, University of Northern Colorado, April 2003.

Brian Keogh, Ryan J. Rafferty, and Richard M. Hyslop, “Synthesis of 6-Mercaptopurine (6-Thiopurine) Analogs.”  Colorado-Wyoming Academy of Sciences, University of Northern Colorado, April 2003.

Melynda M. Carter, Ryan J. Rafferty and Richard M. Hyslop, “Analysis of in vivo Hepatic Concentrations of UDP-Glucose and Glucuronic Acid.” Colorado-Wyoming Academy of Sciences, University of Northern Colorado, April 2003.

Ryan J. Rafferty, “Analysis of the Effects of 6-Thiopurine Analogs on UDP-Glucose Dehydrogenase.”  Departmental Seminar, Department of Chemistry and Biochemistry, University of Northern Colorado, April 2003.

Ryan J. Rafferty and Richard M. Hyslop, “Development of An Analytical Method for the Determination of Enzymatic Activity of UDP-Glucose Dehydrogenase.”  17th Rocky Mountain Regional ACS Meeting, Albuquerque, NM, October 2002. 

Ryan J. Rafferty and Richard M. Hyslop, “Synthesis of 6-Thiopurine Analogs and Their Inhibitory Activity Towards UDP-Glucose Dehydrogenase.”  17th Rocky Mountain Regional ACS Meeting, Albuquerque, NM, October 2002. 

Ryan J. Rafferty, “UDP-Galactose 4-Epimerase: Interconversion of Galactose to Glucose.”  Departmental Seminar, Department of Chemistry and Biochemistry, University of Northern Colorado, October 2002.

Ryan J. Rafferty, “Safety Protocols for Graduate Students Teaching in University Laboratories.”  University of Northern Colorado, Greeley, CO, August 2002.

Poster Presentations

Ryan J. Rafferty and Richard M. Hyslop, “Inhibitory Analysis of 6-Thiopurine and its active Metabolites towards UDP-Glucose Dehydrogenase”  229th National ACS Meeting, San Diego, CA, March 2005.

Nevin R. Gallagher, Ryan J. Rafferty, and Richard M. Hyslop, “Utilization of a Capillary Electrophoretic Method for the Quantification of 6-Thiopurine Metabolites”  229th National ACS Meeting, San Diego, CA, March 2005.

Ryan J. Rafferty and Richard. M. Hyslop, “Methodologies for the Analysis of UDP-Glucuronic Acid In Vitro and In Vivo Utilizing HPLC and LC/MS.”  HPLC Symposium 2004, Philadelphia, PA, June 2004.

Ryan J. Rafferty and Richard. M. Hyslop, “Assessment of Dehydrogenase Enzyme Activities by RP-HPLC.”  HPLC Symposium 2004, Philadelphia, PA, June 2004.

Ryan J. Rafferty and Richard M. Hyslop, “Methodology for the Assessment of UDP-Glucose Dehydrogenase and Crude Synthesis of 6-Thiopurine Analogs.”  UNC Research Day, University of Northern Colorado, April 2004.

Ryan J. Rafferty and Richard M. Hyslop, “HPLC and LC/MS Methods for the Detection and Quantification of UDP-Glucuronic Acid.”  227th National ACS Meeting, Anaheim, CA, March 2004.

Nevin R. Gallagher, Ryan J. Rafferty, and Richard M. Hyslop, “Analysis of 6-Thiopurine Metabolites by Capillary Electrophoresis.”  227th National ACS Meeting, Anaheim, CA, March 2004.

J. Carl Cooley, Ryan J. Rafferty, and Richard M. Hyslop, “Quantification of 6-Thiopurine and   6-Thiouric Acid in Serum and Homogenized Liver Samples.”  227th National ACS Meeting, Anaheim, CA, March 2004.

Hannah C. Robus, Ryan J. Rafferty, and Richard M. Hyslop, “Inhibition of UDP-Glucose Dehydrogenase Activity by 6-Thiopurine Analogs.”  227th National ACS Meeting, Anaheim, CA, March 2004.

Brian H. Keogh, Ryan J. Rafferty, and Richard M. Hyslop, “Synthesis of 6-Thiopurine Analogs.”  227th National ACS Meeting, Anaheim, CA, March 2004.

Ryan J. Rafferty and Richard M. Hyslop, “Method Development for An Assay for UDP-Glucose Dehydrogenase.”  UNC Research Day, University of Northern Colorado, April 2003.

Ryan J. Rafferty and Richard M. Hyslop, “Development of an Analytical Method for the Determination of Enzymatic Activity of UDP-Glucose Dehydrogenase and Inhibitory Activity of Synthesized 6-Thiopurine Analogs.”  225th National ACS Meeting, New Orleans, LA, March 2003.

Publications

“Novel Excel Program to Illustrate the Effects of Mutations Within DNA and on Protein Synthesis”  (Submitted-Under revision)

“In vitro Methodology for the Quantification of UDP-Glucuronic Acid via HPLC with UV Detection”  (In Progress)

“Noncompetitive Inhibition of 6-Thiopurine and its metabolite 6-Thiouric acid towards UDP-Glucose Dehydrogenase”  (Submitted)

“The Determination of in vitro Enzymatic Activity of UDP-Glucose Dehydrogenase”  (In Progress)

“Operational Laboratory Safety Plan: Department of Chemistry and Biochemistry”  (In House Publication)

Hyslop, R.M, Tak-yau, T., and Rafferty, R. J. “Laboratory Manual for Experimental Biochemistry
and Biochemical Techniques.”  University of Northern Colorado, Greeley, CO (2003).